PET28a+_BrCas12b
(Plasmid
#170819)
-
PurposeBacterial expression of BrCas12b
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 170819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepET28+
-
Backbone manufacturerTwist Bioscience
- Backbone size w/o insert (bp) 5202
- Total vector size (bp) 8601
-
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBrCas12b
-
SpeciesBrevibacillus sp. SYP-B805
-
Insert Size (bp)3270
-
GenBank IDWP_165214399.1
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis, T7, Thrombin site (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TAATACGACTCACTATAGG
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.medrxiv.org/content/10.1101/2021.10.15.21265066v1 for medRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PET28a+_BrCas12b was a gift from Piyush Jain (Addgene plasmid # 170819) -
For your References section:
A Thermostable Cas12b from Brevibacillus Leverages One-pot Detection of SARS-CoV-2 Variants of Concern. Nguyen LT, Macaluso NC, Pizzano BLM, Cash MN, Spacek J, Karasek J, Dinglasan RR, Salemi M, Jain PK. medRxiv 10.1101/2021.10.15.21265066
Map uploaded by the depositor.
