|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17177||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
Growth in Bacteria
Gene/Insert namenacre promoter
SpeciesD. rerio (zebrafish)
Insert Size (bp)800
MutationAll three Tcf/Lef sites are mutated
Entrez Genemitfa (a.k.a. nacre, z3A.1)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Description: zebrafish nacre promoter with all three LEF sites mutated, driving luciferase reporter. This is a control construct.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M27 NacMS>luc was a gift from Randall Moon (Addgene plasmid # 17177 ; http://n2t.net/addgene:17177 ; RRID:Addgene_17177)
Map uploaded by the depositor.