PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||173143||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2580
- Total vector size (bp) 3669
Vector typePCR cloning vector
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1089
Entrez Genekcnj13 (a.k.a. ja, kir7, kir7.1, ob)
- Promoter No promoter at 5 end; T7 promoter at 3 end.
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This gene was cloned from a pool of mixed zebrafish embryos (1-3dpf) using RT-PCR.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTR-D-kcnj13 was a gift from GuangJun Zhang (Addgene plasmid # 173143 ; http://n2t.net/addgene:173143 ; RRID:Addgene_173143)
For your References section:Evolution of inwardly rectifying potassium channels and their gene expression in zebrafish embryos. Silic MR, Murata SH, Park SJ, Zhang G. Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.425. 10.1002/dvdy.425 PubMed 34558132