pJK01_Rho_minprox_DsREd
(Plasmid
#173489)
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PurposePCR template for reporter gene with mouse Rhodopsin promoter with DsRed
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAGGS
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Backbone manufacturern/a
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 4015
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Modifications to backbonenone
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Rhodopsin promoter driving DsRed reporter gene
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gRNA/shRNA sequenceATGGCCTCCTCCGAGAACGTCATCACCGAGTTCATGCGCTTCAAGGTGCGCATGGAGGGCACCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCCACAACACCGTGAAGCTGAAGGTGACCAAGGGCGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCCCAGTTCCAGTACGGCTCCAAGGTGTACGTGAAGCACCCCGCCGACATCCCCGACTACAAGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGCGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCTGCTTCATCTACAAGGTGAAGTTCATCGGCGTGAACTTCCCCTCCGACGGCCCCGTGATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGCCTGTACCCCCGCGACGGCGTGCTGAAGGGCGAGACCCACAAGGCCCTGAAGCTGAAGGACGGCGGCCACTACCTGGTGGAGTTCAAGTCCATCTACATGGCCAAGAAGCCCGTGCAGCTGCCCGGCTACTACTACGTGGACGCCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAGCAGTACGAGCGCACCGAGGGCCGCCACCACCTGTTCCTGTA
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SpeciesSynthetic
- Promoter Mouse Rhodopsin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site EagI (unknown if destroyed)
- 5′ sequencing primer CTAGAATGTCACCTTGGCCCC
- 3′ sequencing primer CCACCACCTGTTCCTGTAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThis construct was originally cloned by co-author Joseph Corbo as described in Hsiau, et al. It was further modified as described in Kwasnieski, et al. Proc Natl Acad Sci U S A 2012 Nov 20;109(47):19498-503. doi: 10.1073/pnas.1210678109.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK01_Rho_minprox_DsREd was a gift from Barak Cohen (Addgene plasmid # 173489 ; http://n2t.net/addgene:173489 ; RRID:Addgene_173489) -
For your References section:
Massively parallel in vivo enhancer assay reveals that highly local features determine the cis-regulatory function of ChIP-seq peaks. White MA, Myers CA, Corbo JC, Cohen BA. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11952-7. doi: 10.1073/pnas.1307449110. Epub 2013 Jul 1. 10.1073/pnas.1307449110 PubMed 23818646
Map uploaded by the depositor.
