pNCS-Flag
(Plasmid #17364)

• Depositing Lab: Stephen Goff
• Publication: Yueh et al J Virol. 2003 Feb . 77(3):1820-9.

Backbone

• Vector backbone: pBR322 modified to include SV40 origin
• Backbone size w/o insert (bp): 2000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MMLV proviral DNA
• Species: Moloney murine leukemia virus
• Insert Size (bp): 8000
• Tag / Fusion Protein:
  • Flag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmids were constructed by introducing point mutations into plasmid vector pNCS. Point mutations were created by overlap extension PCR with pNCS template DNA by using outside primers (forward primer in MA region, 5′-CTGGGTCAAGCCCTTTGTACACCCTAAGCC-3′; reverse primer in CA, 5′-GTCTGGGCGCTCGAGGGGAAAAGCG-3′). The sense strand primer utilized for creating mutations was as follows, and the antisense primer was its complement: P12/Flag, 5′-TACAAAGACGATGACGACAAGCCTGCGGGAGAGGCACCG-3'