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pDest490-OPN-c
(Plasmid #17592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17592 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 17592-D50 50 μg of DNA in Tris buffer $495
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DNA 17592-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDest490
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPN-c
  • Alt name
    Osteopontin
  • Alt name
    OPN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    860
  • Mutation
    Base pairs 1–93, 175–942
  • GenBank ID
    X13694
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

OPN-c is missing the amino-terminus exon 4 (27 amino acids).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest490-OPN-c was a gift from Xin Wang (Addgene plasmid # 17592 ; http://n2t.net/addgene:17592 ; RRID:Addgene_17592)

  • For your References section:

    An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW. Oncogene. 2007 Sep 27. 26(44):6361-71. 10.1038/sj.onc.1210463 PubMed 17452979
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