pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169
(Plasmid
#178528)
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PurposeExpresses rtTA and SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Institute for Basic Biology
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 178528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 178528-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 178528-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPB (piggyBac transposase donor vector)
- Backbone size w/o insert (bp) 3450
- Total vector size (bp) 9775
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namertTA, SspB-mScarlet-I-MYPT (1-169aa)
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Alt namertTA2S-M2-G72P-SE
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SpeciesH. sapiens (human), Synthetic
- Promoter CAG, Tet responsive element
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCAG-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The sequence and plasmid map is available in the following benchling link (https://benchling.com/s/seq-xf8zdl8VLNsyQHRmCXqC)
DNA (Catalog # 178528-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 178528-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPBbsr2-rtTA2-TRE-SspB-mScarlet-I-MYPT169 was a gift from Kazuhiro Aoki (Addgene plasmid # 178528 ; http://n2t.net/addgene:178528 ; RRID:Addgene_178528) -
For your References section:
Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Yamamoto K, Miura H, Ishida M, Mii Y, Kinoshita N, Takada S, Ueno N, Sawai S, Kondo Y, Aoki K. Nat Commun. 2021 Dec 8;12(1):7145. doi: 10.1038/s41467-021-27458-3. 10.1038/s41467-021-27458-3 PubMed 34880255
