Purposemammalian expression of human SEPT9_i3 fused to GFP11
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||180346||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
- Promoter CMV
/ Fusion Protein
- GFP11 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://doi.org/10.1101/2022.02.23.481653 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV_SEPT9_i3-14-GFP11 was a gift from Manos Mavrakis (Addgene plasmid # 180346 ; http://n2t.net/addgene:180346 ; RRID:Addgene_180346)
For your References section:Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S, Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, Koenderink GH, Wenger J, Cabantous S, Mavrakis M. J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 10.1083/jcb.202203016. Epub 2022 Dec 23. 10.1083/jcb.202203016 PubMed 36562751