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XRE-pNL1.3[secNLuc]
(Plasmid #182295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182295 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 182295-D50 50 μg of DNA in Tris buffer $495
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DNA 182295-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pNL1.3[secNluc]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3189
  • Total vector size (bp) 4835
  • Modifications to backbone
    XRE sequence was added
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xenobiotic Response Element
  • Alt name
    XRE
  • Alt name
    CYP1A1 promotor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1646
  • Entrez Gene
    CYP1A1 (a.k.a. AHH, AHRR, CP11, CYP1, CYPIA1, P1-450, P450-C, P450DX)
  • Promoter XRE
  • Tag / Fusion Protein
    • secNluc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XRE-pNL1.3[secNLuc] was a gift from Severine Degrelle & Thierry Fournier (Addgene plasmid # 182295 ; http://n2t.net/addgene:182295 ; RRID:Addgene_182295)

  • For your References section:

    Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR). Degrelle SA, Ferecatu I, Fournier T. Environ Int. 2022 Sep 24;169:107545. doi: 10.1016/j.envint.2022.107545. 10.1016/j.envint.2022.107545 PubMed 36179647
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