pKSB-AeU6-3gRNA
(Plasmid #183914)

• Purpose: gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter
• Depositing Lab: Eric Marois
• Publication: Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7.

Backbone

• Vector backbone: pKSB- (Addgene #62540)
• Backbone size w/o insert (bp): 2961

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA scaffold for protospacer cloning into BbsI sites
• Promoter: Aedes aegypti U6 promoter (AAEL017763)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (Golden Gate Cloning) (not destroyed)
• 3′ cloning site: BsaI (Golden Gate Cloning) (not destroyed)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • 1077 pKSB- gBlock Aedes U6gRNA3 AGAG AACA clone5 (1).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

to express a gRNA in Aedes aegypti. Module can be incorporated in a GoldenGate assembly using the BsaI sites. Please visit https://doi.org/10.1101/2022.03.11.483912 for bioRxiv preprint.