pYMA3_SidK_GFP
(Plasmid
#184276)
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PurposeLegionella pneumophila SidK (1-278) - GFP
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Depositing Lab
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Depositing OrganizationHospital for Sick Children
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 184276-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 184276-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N1
- Total vector size (bp) 5544
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSidK (1-278)
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SpeciesLegionella pneumophila
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Insert Size (bp)834
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Entrez GenesidK (a.k.a. A9E85_RS04790, A9E85_04790)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 184276-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 184276-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYMA3_SidK_GFP was a gift from John Rubinstein (Addgene plasmid # 184276 ; http://n2t.net/addgene:184276 ; RRID:Addgene_184276) -
For your References section:
Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK. Maxson ME, Abbas YM, Wu JZ, Plumb JD, Grinstein S, Rubinstein JL. J Cell Biol. 2022 Mar 7;221(3). pii: 212963. doi: 10.1083/jcb.202107174. Epub 2022 Jan 13. 10.1083/jcb.202107174 PubMed 35024770
