mitoTALECD for_atp1_1333CN
(Plasmid #186201)

• Purpose: For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
• Depositing Lab: Shin-ichi Arimura
• Publication: Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;119(20):e2121177119. doi: 10.1073/pnas.2121177119. Epub 2022 May 13.

Backbone

• Vector backbone: pK7WG2
• Backbone manufacturer: VIB, Ghent University
• Total vector size (bp): 22426
• Modifications to backbone: RPS5A promoter, Oleosin-GFP marker added in between Left and right border of T-DNA.
• Vector type: Synthetic Biology ; Ti plasmid for plant transformation
• Selectable markers: Kanamycin and seed GFP fluorescence by OleosinGFP for plant transformation

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
• Alt name: for specific base editing of the RNA editing site C of ATP1 by OTP87
• Species: Synthetic
• Insert Size (bp): 3261
• GenBank ID:

Gene/Insert 2

• Gene/Insert name: mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
• Alt name: for specific base editing of the RNA editing site C of ATP1 by OTP87
• Insert Size (bp): 3117

Resource Information

• Supplemental Documents:
  • mitoTALECD for_atp1_1333CN.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry