VPS13C^halo∆1235-1748
(Plasmid
#187297)
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PurposeExpress VPS13C truncation mutant in mammalian cells
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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187297 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 187297-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 187297-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 14576
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructions32 degree is better to avoid mutagenesis
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVPS13C truncation mutant
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SpeciesH. sapiens (human)
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Insert Size (bp)10629
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Entrez GeneVPS13C (a.k.a. BLTP5C, PARK23)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA plasmid containing codon-optimized cDNA encoding full-length human VPS13C, with a Halo protein after amino acid residue 1914 (VPS13C^Halo), was generated by and purchased from GenScript Biotech
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 187297-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 187297-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VPS13C^halo∆1235-1748 was a gift from Pietro De Camilli (Addgene plasmid # 187297 ; http://n2t.net/addgene:187297 ; RRID:Addgene_187297) -
For your References section:
In situ architecture of the lipid transport protein VPS13C at ER-lysosome membrane contacts. Cai S, Wu Y, Guillen-Samander A, Hancock-Cerutti W, Liu J, De Camilli P. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2203769119. doi: 10.1073/pnas.2203769119. Epub 2022 Jul 13. 10.1073/pnas.2203769119 PubMed 35858323
