G12-CASE
(Plasmid
#190714)
-
PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
-
Depositing Lab
-
Depositing OrganizationKarolinska Institutet
Located in Sweden -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 190714-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 190714-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneClontech-style N1
-
Backbone manufacturerClontech
-
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameGNB3
-
SpeciesH. sapiens (human)
-
Entrez GeneGNB3 (a.k.a. CSNB1H, HG2D)
Gene/Insert 2
-
Gene/Insert nameGNGT9
-
SpeciesH. sapiens (human)
-
Tag
/ Fusion Protein
- cpVenus (N terminal on insert)
Gene/Insert 3
-
Gene/Insert nameGNA12
-
SpeciesH. sapiens (human)
-
Entrez GeneGNA12 (a.k.a. HG1M1, NNX3, RMP, gep)
-
Tag
/ Fusion Protein
- Nluc is inserted at A133/F134 within GNA12
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 190714-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 190714-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
G12-CASE was a gift from Gunnar Schulte (Addgene plasmid # 190714 ; http://n2t.net/addgene:190714 ; RRID:Addgene_190714) -
For your References section:
Isoforms of GPR35 have distinct extracellular N-termini that allosterically modify receptor-transducer coupling and mediate intracellular pathway bias. Schihada H, Klompstra TM, Humphrys LJ, Cervenka I, Dadvar S, Kolb P, Ruas JL, Schulte G. J Biol Chem. 2022 Aug 3:102328. doi: 10.1016/j.jbc.2022.102328. 10.1016/j.jbc.2022.102328 PubMed 35933013
