hPCNA-C148S
(Plasmid
#190939)
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Purposeuntagged human PCNA with a cysteine to serine mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET3c
- Backbone size w/o insert (bp) 4638
- Total vector size (bp) 5421
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameProliferating Cell Nuclear Antigen
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Alt namePCNA
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SpeciesH. sapiens (human)
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Insert Size (bp)783
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Mutationchanged cysteine 148 to serine
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Entrez GenePCNA (a.k.a. ATLD2)
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byunknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hPCNA-C148S was a gift from Brian Kelch (Addgene plasmid # 190939 ; http://n2t.net/addgene:190939 ; RRID:Addgene_190939)