pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR
(Plasmid #192939)

• Purpose: FlpO-based digitizer circuit driven by AP1 promoter
• Depositing Lab: Wilson Wong
• Depositing Organization: Boston University Main Campus
  Located in United States of America
• Publication: Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi: 10.1038/s41467-021-26359-9.

Backbone

• Vector backbone: pAAVS1
• Total vector size (bp): 15397
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: AP1(2)/minTK promoter
• Insert Size (bp): 142

Gene/Insert 2

• Gene/Insert name: FlpO-ABI
• Insert Size (bp): 2166

Gene/Insert 3

• Gene/Insert name: PYL-FlpO
• Insert Size (bp): 708

Gene/Insert 4

• Gene/Insert name: CAG promoter
• Insert Size (bp): 1701

Gene/Insert 5

• Gene/Insert name: frt-STOP-frt
• Insert Size (bp): 1227

Gene/Insert 6

• Gene/Insert name: GFP
• Insert Size (bp): 717

Gene/Insert 7

• Gene/Insert name: luciferase
• Insert Size (bp): 1653

Gene/Insert 8

• Gene/Insert name: pPGK
• Insert Size (bp): 504

Gene/Insert 9

• Gene/Insert name: Zeocin resistance
• Insert Size (bp): 372

Gene/Insert 10

• Gene/Insert name: BFP
• Insert Size (bp): 696

Resource Information

• A portion of this plasmid was derived from a plasmid made by: AP1(2) short response element sequence is from Affymetrix.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 192939-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 192939-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.