pML3009
(Plasmid #193256)

• Purpose: Gamma-aminobutyric acid sensor expressing eYFP reporter
• Depositing Lab: Lauren Andrews
• Publication: Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:938056. doi: 10.3389/fbioe.2022.938056. eCollection 2022.

Backbone

• Vector backbone: pAN1201
• Total vector size (bp): 6655
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: gabR
• Insert Size (bp): 1439
• Promoter: synthetic promoter P2

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SapI (destroyed during cloning)
• 3′ cloning site: SapI (destroyed during cloning)
• 5′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: eYFP
• Insert Size (bp): 720
• Promoter: synthetic promoter PGab105

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: TGGCTCATAACACCCCTTG

Resource Information

• Supplemental Documents:
  • pML3009.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry