pCin4-3xFLAG-MKL1 S449A/T450A/S454A
(Plasmid
#19845)
-
Depositing Lab
-
Depositing OrganizationColumbia University
Located in United States of America -
Citations
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 19845-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 19845-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepCin4
-
Backbone manufacturerS Rees et al., BioTechniques 1996
- Backbone size w/o insert (bp) 5257
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMKL1 S449A/T450A/S454A
-
Alt nameMAL, BSAC, MRTF-A
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2800
-
Mutationchanged Serine 449, Threonine 450 and Serine 454 to Alanine
-
Entrez GeneMRTFA (a.k.a. BSAC, MAL, MKL, MKL1, MRTF-A)
-
Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 3xFlag in the vector is from Sigma, p3xFlag-CMV-7.1 has variant flag sequences (DYKDHD) and is recognized well by their anti-flag monoclonal M2.
DNA (Catalog # 19845-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 19845-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCin4-3xFLAG-MKL1 S449A/T450A/S454A was a gift from Ron Prywes (Addgene plasmid # 19845 ; http://n2t.net/addgene:19845 ; RRID:Addgene_19845) -
For your References section:
Serum-induced phosphorylation of the serum response factor coactivator MKL1 by the extracellular signal-regulated kinase 1/2 pathway inhibits its nuclear localization. Muehlich S, Wang R, Lee SM, Lewis TC, Dai C, Prywes R. Mol Cell Biol. 2008 Oct;28(20):6302-13. doi: 10.1128/MCB.00427-08. Epub 2008 Aug 11. 10.1128/MCB.00427-08 PubMed 18694962
Map uploaded by the depositor.
