pSEMS-Tom20-mEGFP-reHaloTagF
(Plasmid
#200190)
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PurposeTom20 with reversible HaloTagF variant for fluorescent labeling
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Depositing Lab
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Depositing OrganizationUniversitaet Osnabrueck
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 200190-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 200190-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSEMS(26m)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTom20
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SpeciesH. sapiens (human)
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Insert Size (bp)435
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Entrez GeneTOMM20 (a.k.a. MAS20, MOM19, TOM20)
- Promoter CMV
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Tags
/ Fusion Proteins
- mEGFP (C terminal on insert)
- reHaloTagF (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer ATGGTGGGTCGGAACAGCGCCAT
- 3′ sequencing primer TTCCACATCATCTTCAGCCAAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 200190-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 200190-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEMS-Tom20-mEGFP-reHaloTagF was a gift from Jacob Piehler (Addgene plasmid # 200190 ; http://n2t.net/addgene:200190 ; RRID:Addgene_200190) -
For your References section:
Reversible Live-Cell Labeling with Retro-engineered HaloTags Enables Long-Term High- and Super-Resolution Imaging. Holtmannspotter M, Wienbeuker E, Dellmann T, Watrinet I, Garcia-Saez AJ, Johnsson K, Kurre R, Piehler J. Angew Chem Int Ed Engl. 2023 Feb 3:e202219050. doi: 10.1002/anie.202219050. 10.1002/anie.202219050 PubMed 36735334
Map uploaded by the depositor.
