pYZ038
(Plasmid
#200227)
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Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPY
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Depositing Lab
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Depositing OrganizationNYU Grossman School of Medicine
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 200227-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 200227-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 3500
- Total vector size (bp) 3673
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructions30°C is recommended and 37°C is also fine
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametRNA-gRNA scaffold
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SpeciesS. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)153
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
DNA (Catalog # 200227-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 200227-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYZ038 was a gift from Jef Boeke (Addgene plasmid # 200227 ; http://n2t.net/addgene:200227 ; RRID:Addgene_200227) -
For your References section:
CREEPY: CRISPR-mediated editing of synthetic episomes in yeast. Zhao Y, Coelho C, Lauer S, Majewski M, Laurent JM, Brosh R, Boeke JD. Nucleic Acids Res. 2023 Jun 16:gkad491. doi: 10.1093/nar/gkad491. 10.1093/nar/gkad491 PubMed 37326023
Map uploaded by the depositor.
