H6SUMO-IntC-P53(304-393)
(Plasmid
#200316)
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Purposebacterial expression of P53 TET-CTD for segmental labeling
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200316 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep53 (304-393)
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SpeciesH. sapiens (human)
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Insert Size (bp)645
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
- Promoter T7
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Tags
/ Fusion Proteins
- His6-SUMO (N terminal on insert)
- designed intein C (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The intein sequence was taken from Addgene #20177
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H6SUMO-IntC-P53(304-393) was a gift from Peter Wright (Addgene plasmid # 200316 ; http://n2t.net/addgene:200316 ; RRID:Addgene_200316) -
For your References section:
Mapping Interactions of the Intrinsically Disordered C-Terminal Regions of Tetrameric p53 by Segmental Isotope Labeling and NMR. Krois AS, Park S, Martinez-Yamout MA, Dyson HJ, Wright PE. Biochemistry. 2022 Dec 6;61(23):2709-2719. doi: 10.1021/acs.biochem.2c00528. Epub 2022 Nov 15. 10.1021/acs.biochem.2c00528 PubMed 36380579
