pDEST40-CES2A-ΔC-S227A
(Plasmid
#200556)
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PurposeMammalian expression of secreted mouse CES2A S227A mutant
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 200556-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 200556-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDEST40
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMouse Carboxylesterase 2A S227A mutant delta C-terminal
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SpeciesM. musculus (mouse)
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Entrez GeneCes2a (a.k.a. 9130231C15Rik, Ces6)
- Promoter CMV promoter
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- 6xHis (C terminal on insert)
- V5
- 6xHis
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 200556-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 200556-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST40-CES2A-ΔC-S227A was a gift from Jonathan Long (Addgene plasmid # 200556 ; http://n2t.net/addgene:200556 ; RRID:Addgene_200556) -
For your References section:
Organism-wide, cell-type-specific secretome mapping of exercise training in mice. Wei W, Riley NM, Lyu X, Shen X, Guo J, Raun SH, Zhao M, Moya-Garzon MD, Basu H, Sheng-Hwa Tung A, Li VL, Huang W, Wiggenhorn AL, Svensson KJ, Snyder MP, Bertozzi CR, Long JZ. Cell Metab. 2023 Apr 28:S1550-4131(23)00138-9. doi: 10.1016/j.cmet.2023.04.011. 10.1016/j.cmet.2023.04.011 PubMed 37141889
Map uploaded by the depositor.
