pYS17
(Plasmid
#202083)
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PurposeParent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in
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Depositing Lab
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Depositing OrganizationUniversity of Texas at Austin
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 202083-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 202083-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneMN100A-1
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Backbone manufacturerSystem Biosciences
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNT PuroR-T2A-mNG Minicircle Parent
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SpeciesSynthetic
- Promoter None
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Tag
/ Fusion Protein
- mNeonGreen (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
IMPORTANT: This plasmid is shipped from Addgene in NEB Stable cells, but these cells are NOT suitable for minicircle production. You will need to purchase ZYCY10P3S2T cells separately, and transform the plasmid into that host strain, to produce minicircles.
DNA (Catalog # 202083-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 202083-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYS17 was a gift from Daniel Dickinson (Addgene plasmid # 202083 ; http://n2t.net/addgene:202083 ; RRID:Addgene_202083) -
For your References section:
Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells. Shi Y, Kopparapu N, Ohler L, Dickinson DJ. Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. 10.1242/dev.201367 PubMed 37129004
