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pGFP-Sirt6
(Plasmid #20274)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20274 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sirt6
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1002
  • GenBank ID
    NM_181586
  • Entrez Gene
    Sirt6 (a.k.a. 2810449N18Rik, Sir2l6, mSIRT6)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP-Sirt6 was a gift from Leonard Guarente (Addgene plasmid # 20274)

  • For your References section:

    Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase. Liszt G, Ford E, Kurtev M, Guarente L. J Biol Chem. 2005 Jun 3. 280(22):21313-20. 10.1074/jbc.M413296200 PubMed 15795229
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