AAV-RO1.7-Hsp90ab1-FLAG
(Plasmid
#206355)
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PurposeAAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 206355 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-RO1.7-W3SL
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHsp90ab1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1248
- Promoter 1.7kb human red opsin
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gggacagggctttccatagcatATGCCTGAGGAAGTGCACCA
- 3′ sequencing primer tgattatctcgagcggGCTActtgtcgtcatcgtctttgt (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-RO1.7-Hsp90ab1-FLAG was a gift from Connie Cepko (Addgene plasmid # 206355 ; http://n2t.net/addgene:206355 ; RRID:Addgene_206355)