pLRD07
              
              
                (Plasmid
                
                #208260)
              
            
            
            
          - 
            PurposeProduction of (16R)-ent-kauran-16-ol in E. coli MEV20
 - 
              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backbonepLRD
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              Vector typeBacterial Expression
 
Growth in Bacteria
- 
            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberLow Copy
 
Gene/Insert 1
- 
                Gene/Insert nametHMGR
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                    SpeciesSaccharomyces cerevisiae
 
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
 - 5′ cloning site unknown (unknown if destroyed)
 
Gene/Insert 2
- 
                Gene/Insert nameptmT2
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                    SpeciesStreptomyces platensis
 
Gene/Insert 3
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                Gene/Insert nameptmT3
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                    SpeciesStreptomyces platensis
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLRD07 was a gift from Christopher Voigt (Addgene plasmid # 208260 ; http://n2t.net/addgene:208260 ; RRID:Addgene_208260) - 
                
For your References section:
Design of a redox-proficient Escherichia coli for screening terpenoids and modifying cytochrome P450s. Lin GM, Voigt CA. Nature Catalysis. 6, 1016–1029 (2023). https://doi.org/10.1038/s41929-023-01049-5 10.1038/s41929-023-01049-5