ABE-RA1.1
              
              
                (Plasmid
                
                #208298)
              
            
            
            
          - 
            PurposeExpresses ABE-RA1.1 in mammalian cells
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              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backbonepCMV with a BR322 origin
 - Backbone size w/o insert (bp) 3402
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              Vector typeMammalian Expression
 
Growth in Bacteria
- 
            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameTadA-RA1.1-SpCas9 D10A
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                    SpeciesE. coli
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                  Insert Size (bp)4818
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                  MutationD108G, K161N
 
Cloning Information
- Cloning method Restriction Enzyme
 - 5′ cloning site Unknown (unknown if destroyed)
 - 3′ cloning site Unknown (unknown if destroyed)
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ABE-RA1.1 was a gift from Weixin Tang (Addgene plasmid # 208298 ; http://n2t.net/addgene:208298 ; RRID:Addgene_208298) - 
                
For your References section:
An adenine base editor variant expands context compatibility. Xiao YL, Wu Y, Tang W. Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01994-3. 10.1038/s41587-023-01994-3 PubMed 38168987