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pCB-2
(Plasmid #210388)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210388 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 210388-D50 50 μg of DNA in Tris buffer $495
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DNA 210388-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pPAP
  • Backbone manufacturer
    Chunbo Lou
  • Backbone size w/o insert (bp) 4985
  • Total vector size (bp) 11711
  • Vector type
    CRISPR ; Integrative vector for use in Streptomyces

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9
  • Alt name
    Codon optimized SpdCas9
  • Insert Size (bp)
    4107
  • Mutation
    The BsaI sites were removed via synonymous mutations
  • Promoter SP30

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CymR
  • Alt name
    CymR repressor
  • Species
    Pseudomonas putida
  • Insert Size (bp)
    616
  • Promoter SP11

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    sgRNA cassette
  • Species
    Synthetic
  • Insert Size (bp)
    1379
  • Mutation
    BsaI-flanked RFP cassette inserted in place of spacer
  • Promoter SP30

Cloning Information for Gene/Insert 3

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received the backbone as a gift from Prof. Chunbo Lou (Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB-2 was a gift from Gilles van Wezel (Addgene plasmid # 210388 ; http://n2t.net/addgene:210388 ; RRID:Addgene_210388)

  • For your References section:

    CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces. Bai C, van Wezel GP. ACS Synth Biol. 2023 Oct 6. doi: 10.1021/acssynbio.3c00464. 10.1021/acssynbio.3c00464 PubMed 37801665
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