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Addgene

pDonorTp
(Plasmid #211788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonor_ShCAST_kanR
  • Backbone manufacturer
    Feng Zhang (Plasmid #127924)

Growth in Bacteria

  • Bacterial Resistance(s)
    Trimethoprim, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dhfr
  • Insert Size (bp)
    671

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonorTp was a gift from Silvia Cardona (Addgene plasmid # 211788 ; http://n2t.net/addgene:211788 ; RRID:Addgene_211788)

  • For your References section:

    A CRISPR-Cas-associated transposon system for genome editing in Burkholderia cepacia complex species. Yap ZL, Rahman ASMZ, Hogan AM, Levin DB, Cardona ST. Appl Environ Microbiol. 2024 Jul 24;90(7):e0069924. doi: 10.1128/aem.00699-24. Epub 2024 Jun 13. 10.1128/aem.00699-24 PubMed 38869300
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