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CaMKII ER-Halo-GCaMP6-150
(Plasmid #216316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216316 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    FCK(1.3)GW
  • Backbone size w/o insert (bp) 9246
  • Total vector size (bp) 11601
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ER-Halo-GCaMP6-150
  • Species
    Synthetic
  • Insert Size (bp)
    2355
  • Promoter CamKII

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer acagtgccctgctcagaagc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene 86918 (ER-GCaMP6-150)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.15.580492 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaMKII ER-Halo-GCaMP6-150 was a gift from Timothy Ryan (Addgene plasmid # 216316 ; http://n2t.net/addgene:216316 ; RRID:Addgene_216316)

  • For your References section:

    A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions. Farrell RJ, Bredvik KG, Hoppa MB, Hennigan ST, Brown TA, Ryan TA. bioRxiv 2024.02.15.580492 10.1101/2024.02.15.580492
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