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MAP4K3-GFP
(Plasmid #21731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21731 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21731-D50 50 μg of DNA in Tris buffer $495
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DNA 21731-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1/CT-GFP-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6157
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP4K3
  • Alt name
    GLK
  • Alt name
    MAPKKKK3
  • Alt name
    RAB8IPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2619
  • GenBank ID
    BC071579
  • Entrez Gene
    MAP4K3 (a.k.a. GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ cloning site T/A cloning (unknown if destroyed)
  • 3′ cloning site T/A cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer GFP Reverse (5 ́-GGGTAAGCTTTCCGTATGTAGC-3 ́)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAP4K3-GFP was a gift from L. Miguel Martins (Addgene plasmid # 21731 ; http://n2t.net/addgene:21731 ; RRID:Addgene_21731)

  • For your References section:

    MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins. Lam D, Dickens D, Reid EB, Loh SH, Moisoi N, Martins LM. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11978-83. doi: 10.1073/pnas.0900608106. Epub 2009 Jul 8. 10.1073/pnas.0900608106 PubMed 19587239
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