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pDEST27-GST-ATXN1 FL(92Q)-S776D
(Plasmid #21757)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21757 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 21757-D50 50 μg of DNA in Tris buffer $495
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DNA 21757-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDEST27
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8123
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH10B or similar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ataxin-1
  • Alt name
    ATXN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2604
  • Mutation
    insert contains a stretch of 92 Q(Glutamines) and no stop codon; Serine 776 changed to Aspartic Acidthis is the mutant (expanded) version of Ataxin-1
  • GenBank ID
    NM_000332
  • Entrez Gene
    ATXN1 (a.k.a. ATX1, D6S504E, SCA1)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer M13 (-21) Forward or T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST27-GST-ATXN1 FL(92Q)-S776D was a gift from Huda Zoghbi (Addgene plasmid # 21757 ; http://n2t.net/addgene:21757 ; RRID:Addgene_21757)

  • For your References section:

    Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1. Lim J, Crespo-Barreto J, Jafar-Nejad P, Bowman AB, Richman R, Hill DE, Orr HT, Zoghbi HY. Nature. 2008 Apr 10. 452(7188):713-8. 10.1038/nature06731 PubMed 18337722
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