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ChR2-2A-Halo
(Plasmid #22047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22047 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 22047-D50 50 μg of DNA in Tris buffer $495
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DNA 22047-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 3963
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChR2-GFP-2A-Halo-YFP
  • Alt name
    NpHR
  • Alt name
    channelrhodopsin
  • Alt name
    halorhodopsin
  • Species
    C. reinhardtii, N. pharaonis
  • Insert Size (bp)
    3356
  • Mutation
    Note that there is a S147R mut in YFP. This mutation is not known to affect fluorescence.
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP and YFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer AGCAGAGCTGGTTTAGTGAA
  • 3′ sequencing primer TAAAACCTCTACAAATGTGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ChR2-2A-Halo was a gift from Edward Boyden (Addgene plasmid # 22047 ; http://n2t.net/addgene:22047 ; RRID:Addgene_22047)

  • For your References section:

    Informational lesions: optical perturbation of spike timing and neural synchrony via microbial opsin gene fusions. Han X, Qian X, Stern P, Chuong AS, Boyden ES. Front Mol Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009 10.3389/neuro.02.012.2009 PubMed 19753326
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