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pSR2009
(Plasmid #222399)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222399 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PPhlF-YFP
  • Backbone size w/o insert (bp) 4311
  • Total vector size (bp) 4306
  • Modifications to backbone
    Replaced eYFP coding sequence with sfGFP coding sequence
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    763
  • Promoter PPhlF

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer aaaaccgaaaagattacttcg
  • 3′ sequencing primer ctctgattttccagtctga
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSR2009 was a gift from Lauren Andrews (Addgene plasmid # 222399 ; http://n2t.net/addgene:222399 ; RRID:Addgene_222399)
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