pMyt1(502-623)
(Plasmid
#22655)
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Depositing Lab
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Depositing OrganizationNational Institute of Neurological Disorders and Strokes (NINDS)
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 22655-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 22655-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBIND
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6360
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyelin Transcription Factor 1
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Alt nameMyt1
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SpeciesM. musculus (mouse)
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Insert Size (bp)364
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Mutationcontains only first 1/2 of Myt1 central domain (CD) 2035-2399bp)
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Entrez GeneMyt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T3 Promoter
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
First 1/2 of Myt1 central domain. bp 2035-2399 and aa 602-623. Used for Mammalian 2-hybrid assays
This Myt1 DNA cloned by Hudson lab.
DNA (Catalog # 22655-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 22655-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMyt1(502-623) was a gift from Lynn Hudson (Addgene plasmid # 22655 ; http://n2t.net/addgene:22655 ; RRID:Addgene_22655) -
For your References section:
Myt1 family recruits histone deacetylase to regulate neural transcription. Romm E, Nielsen JA, Kim JG, Hudson LD. J Neurochem. 2005 Jun . 93(6):1444-53. 10.1111/j.1471-4159.2005.03131.x PubMed 15935060
Map uploaded by the depositor.
