pLH121
(Plasmid
#22696)
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Depositing Lab
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Depositing OrganizationNational Institute of Neurological Disorders and Strokes (NINDS)
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 22696-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 22696-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTZ19R
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Backbone manufacturerFermentas
- Backbone size w/o insert (bp) 2862
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Vector typePhagemid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha (normal growth conditiions)
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman Proteolipid Protein cDNA
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Alt namePLP
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SpeciesH. sapiens (human)
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Insert Size (bp)9000
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Entrez GenePLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer M13/pUC (-20)
- 3′ sequencing primer M13/pUC reverse
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe human PLP genomic and cDNA was cloned in Hudson lab
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This was the PLP transgene construct: consists of the human PLP cDNA, flanked on the 5' side by 4.2 kb of PLP promoter sequence from the human genomic DNA and on the 3' side by 1.5 kb of genomic DNA from the 3' end of the human PLP gene.
DNA (Catalog # 22696-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 22696-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLH121 was a gift from Lynn Hudson (Addgene plasmid # 22696 ; http://n2t.net/addgene:22696 ; RRID:Addgene_22696) -
For your References section:
A combination of PLP and DM20 transgenes promotes partial myelination in the jimpy mouse. Nadon NL, Arnheiter H, Hudson LD. J Neurochem. 1994 Sep . 63(3):822-33. 10.1046/j.1471-4159.1994.63030822.x PubMed 7519661