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Addgene

pmMyt1
(Plasmid #22713)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22713 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3948
  • Vector type
    cloning vector TT/AA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Normal growth conditions
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myelin Transcription Factor 1
  • Alt name
    Myt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5466
  • Entrez Gene
    Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA cloned in Hudson laboratory
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The murine Myt1 cDNA (6 zinc fingers) had 3 base mutations within the coding region (bp 806, 837 and 4344). These sites were corrected using Stratagene QuickChang Site-Directed mutagenesis. The Myt1 was sequenced after the mutagenesis and found to be correct. There is still one mutation in the 5' UTR at bp 529 (START codon is bp 532)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmMyt1 was a gift from Lynn Hudson (Addgene plasmid # 22713 ; http://n2t.net/addgene:22713 ; RRID:Addgene_22713)
  • For your References section:

    Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. J Neurosci Res. 1997 Oct 15. 50(2):272-90. 10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A PubMed 9373037