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pCAG::LSL::tauLacZ
(Plasmid #22848)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22848 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression ; gene targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tauLacZ
  • Species
    bovine / E. coli
  • Insert Size (bp)
    4300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also has Kan resistance

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG::LSL::tauLacZ was a gift from Robert Benezra (Addgene plasmid # 22848)

  • For your References section:

    High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442
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