EGFP_SeV_RdRP
(Plasmid
#228808)
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PurposeExpression of Sendai virus RdRP
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Depositing Lab
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Depositing OrganizationInstitute of Genomics and Integrative Biology (CSIR-IGIB)
Located in India -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 228808-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 228808-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
- Backbone size w/o insert (bp) 4733
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRdRP
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Alt nameLarge
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SpeciesSendai virus
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Insert Size (bp)6687
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA from infected cells
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.01.564969 for bioRxiv preprint.
DNA (Catalog # 228808-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 228808-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP_SeV_RdRP was a gift from Shravanti Rampalli (Addgene plasmid # 228808 ; http://n2t.net/addgene:228808 ; RRID:Addgene_228808) -
For your References section:
A cytoplasmic form of EHMT1N methylates viral proteins to enable inclusion body maturation and efficient viral replication. Biligiri KK, Sharma NR, Mohanty A, Sarkar DP, Vemula PK, Rampalli S. PLoS Biol. 2024 Nov 7;22(11):e3002871. doi: 10.1371/journal.pbio.3002871. eCollection 2024 Nov. 10.1371/journal.pbio.3002871 PubMed 39509467
