pGEM-7Zf-wild
(Plasmid #23160)

• Depositing Lab: SeWon Suh
• Publication: Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Backbone

• Vector backbone: pGEM-7Zf-wild
• Backbone size w/o insert (bp): 2997
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ribonuclease assay sub1
• Insert Size (bp): 188

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a small gap in alignment between depositor's sequence and Addgene's sequence with T7 primer.
The discrepancy is due to a very stable stem loop, which makes it difficult to do DNA sequencing without cutting it with a restriction enzyme. You need to cut it at a PstI site in the loop region before doing DNA sequencing.