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pET28a-AscC-L180F
(Plasmid #232333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232333 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6297
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mutant prenyltransferase
  • Alt name
    AscC-L180F
  • Insert Size (bp)
    1001
  • Mutation
    changed Leucine 180 to Phenylalanine
  • Entrez Gene
    ubiX (a.k.a. b2311, ECK2305, dedF)
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-AscC-L180F was a gift from Shenghong Li & Yan Liu (Addgene plasmid # 232333 ; http://n2t.net/addgene:232333 ; RRID:Addgene_232333)

  • For your References section:

    Engineering a promiscuous prenyltransferase for selective biosynthesis of an undescribed bioactive cannabinoid analog. Yan Q, Chen YG, Yang XW, Wang A, He XP, Tang X, Hu H, Guo K, Xiao ZH, Liu Y, Li SH. Commun Biol. 2025 Feb 4;8(1):173. doi: 10.1038/s42003-025-07509-x. 10.1038/s42003-025-07509-x PubMed 39905239
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