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pCep4β-CyPrP (23-231) V180I (Met 129)
(Plasmid #23307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23307 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCep4β
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10188
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5α in LB
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human Prion Protein
  • Alt name
    hPrP
  • Alt name
    PRNP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    632
  • Mutation
    V180I, Met 129 Polymorphism, Deleted ER Signal Peptide and GPI Anchor Signal Peptide sequences to produce cytosolic PrP (AA 23-231)
  • Entrez Gene
    PRNP (a.k.a. ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference:
Jodoin J, Laroche-Pierre S, Goodyer CG, LeBlanc AC.(2007) J Neurosci.27:5081-91.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCep4β-CyPrP (23-231) V180I (Met 129) was a gift from Andrea LeBlanc (Addgene plasmid # 23307)

  • For your References section:

    Defective retrotranslocation causes loss of anti-Bax function in human familial prion protein mutants. Jodoin J, Laroche-Pierre S, Goodyer CG, LeBlanc AC. J Neurosci. 2007 May 9. 27(19):5081-91. 10.1523/JNEUROSCI.0957-07.2007 PubMed 17494694
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