miR3 MmATP13A4
(Plasmid
#233705)
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Purposetransfer plasmid for lentiviral vector production with miR for Mm ATP13A4
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 233705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneSIV transfer plasmid
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Backbone manufacturerDidier Negre
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Vector typeLentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemiR Atp13a4
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gRNA/shRNA sequence5'-AGCCCATGAACTTCAAGCTCTA-3'
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SpeciesM. musculus (mouse)
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Entrez GeneAtp13a4 (a.k.a. 4631413J11Rik, 4832416L12, 9330174J19Rik)
- Promoter SFFV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Esp3I (destroyed during cloning)
- 3′ cloning site Esp3I (destroyed during cloning)
- 5′ sequencing primer TCATTTTACTGGGGGACCTTGTGCA
- 3′ sequencing primer caacgggccacaactcctc
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR3 MmATP13A4 was a gift from Veerle Baekelandt (Addgene plasmid # 233705)