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pcDNA3.1 Micall2-HA
(Plasmid #238507)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238507 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1
  • Total vector size (bp) 8089
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MICAL-like protein 2
  • Alt name
    MICALL2
  • Alt name
    JRAB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2712
  • Entrez Gene
    MICALL2 (a.k.a. JRAB, MICAL-L2)
  • Tag / Fusion Protein
    • HA-tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer TGACACCTACTCAGACAATGC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene derived from pDONR211 vector (DNASU, catalog #HsCD00296916)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation in original Plasmid from DNASU V85A mutated back to valine
Primer fw: ACATGGtGGCCTTGAAGGTGCCTGAC
Primer rev: AAGGCCaCCATGTCCTCGGCATCCAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 Micall2-HA was a gift from Florian Stengel (Addgene plasmid # 238507 ; http://n2t.net/addgene:238507 ; RRID:Addgene_238507)

  • For your References section:

    Probing the proteome-wide impact of inhibitors of Leucine-rich Repeat Kinases 1 and 2 on protein-protein interactions and phosphorylation. Jansen J, Wendel M, Raig ND, Kraus T, Surridge KJ, Mahesula S, Richter-Müller N, Mathea S, Reck-Peterson SL, Knapp S, Stengel F. bioRxiv 2025.09.03.674114 10.1101/2025.09.03.674114
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