CaMK2rep-S603A
(Plasmid #239623)

• Purpose: Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
• Depositing Lab: F Javier Díez Guerra
• Depositing Organization: Universidad Autonoma de Madrid
  Located in Spain
• Publication: Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-20290. doi: 10.1021/acs.analchem.5c03227. Epub 2025 Sep 14.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5481
• Total vector size (bp): 6661
• Modifications to backbone: EM7 promoter-BleoR instead of NeoR/KanR
• Vector type: Mammalian Expression
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SpotNES-sGFP2-SynP3-3xmyc
• Species: R. norvegicus (rat), Synthetic
• Insert Size (bp): 1236
• Mutation: changed Serine 603 to Alanine
• GenBank ID: NM_019133.2
• Entrez Gene: Syn1
• Promoter: CMV
• Tags / Fusion Proteins:
  • Spot (N terminal on insert)
  • sGFP2 (N terminal on insert)
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTTTGACTCACGGGGATTTCCAAGTC
• 3′ sequencing primer: CCCACCCCACCCCCCAGAATAGAATGACA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.
Depositor confirms P89S in sGFP2, changes are not expected to impact function.

DNA (Catalog # 239623-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 239623-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.