pET15Sumo2-Polio3C_GTM_001
(Plasmid #240387)

• Purpose: Expresses Polio virus protease in E. coli
• Depositing Lab: Gaetano Montelione
• Publication: A PA CLAN PROTEASE CROSS-INHIBITION SCREEN (unpublished)

Backbone

• Vector backbone: pET15SUMO2
• Backbone manufacturer: Novagen/NESG
• Backbone size w/o insert (bp): 5681
• Total vector size (bp): 6235
• Modifications to backbone: An N-terminal 6X-His tag fused to the Smt3 gene (Sumo) of Saccharomyces cerevisiae as a purification/solubility enhancement cassette. BsaI sites are included in the cloning polylinker for scarless cloning and the production of a native, mature protein after cleavage from the Sumo fusion by Sumo protease (Ulp1).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in BL21 DE3 cells for expression
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 3C protease
• Species: Human poliovirus 1 Mahoney
• Insert Size (bp): 554
• GenBank ID:
• Promoter: T7 lac
• Tag / Fusion Protein:
  • 6xHis, SUMO (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Construct purchased from Genscript USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Related to PDB: 1L1N