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pCMVHA E2F4
(Plasmid #24228)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24228 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCMVHANeoBam
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2F4
  • Alt name
    E2F-4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    E2F4 (a.k.a. E2F-4)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer Bglob-intron-F
  • 3′ sequencing primer Bglob-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E2F4 cDNA with HA-tag excised from pTETE2F4 as an EcoRI/BamHI fragment. Test with SalI leads to 800bp + 7,8kb

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVHA E2F4 was a gift from Kristian Helin (Addgene plasmid # 24228)

  • For your References section:

    Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression. Lukas J, Petersen BO, Holm K, Bartek J, Helin K. Mol Cell Biol. 1996 Mar . 16(3):1047-57. PubMed 8622649
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