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pKMM020
(Plasmid #242508)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242508 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pK18mobsacB
  • Backbone manufacturer
    ATCC® 87097
  • Backbone size w/o insert (bp) 5330
  • Total vector size (bp) 10978

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Upstream homology arm
  • Species
    Pseudomonas putida KT2440
  • Insert Size (bp)
    725

Gene/Insert 2

  • Gene/Insert name
    OphC_AG8816
  • Species
    Stutzerimonas stutzeri KGS-2
  • Insert Size (bp)
    993
  • Promoter tac

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    OphC2_AG8816
  • Species
    Stutzerimonas stutzeri KGS-2
  • Insert Size (bp)
    849

Gene/Insert 4

  • Gene/Insert name
    OphK_AG8816
  • Species
    Stutzerimonas stutzeri KGS-2
  • Insert Size (bp)
    1356
  • Promoter Ptac

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    Downstream homology arm
  • Species
    Pseudomonas putida KT2440
  • Insert Size (bp)
    777

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKMM020 was a gift from Gregg Beckham (Addgene plasmid # 242508 ; http://n2t.net/addgene:242508 ; RRID:Addgene_242508)

  • For your References section:

    Lignin to adipic acid in a high-yield chemical and biological redox process. Mains KM, Palumbo CT, Rigo D, Webber MS, Rosetto G, Bao ST, Carroll AL, Meyer NR, Benson AF, Boyle BA, Haugen SJ, Ingraham MA, Alexander WG, Silberman M, Myers LC, Ramirez KJ, Sullivan KP, Guss AM, Salvachua D, Roman-Leshkov Y, Stahl SS, Werner AZ, Beckham GT. Nature. 2026 Jun;654(8119):668-675. doi: 10.1038/s41586-026-10580-x. Epub 2026 Jun 10. 10.1038/s41586-026-10580-x PubMed 42271049
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