Skip to main content
Addgene
Open Menu

CROPseq-V2-Puro.P2A.mCherry
(Plasmid #242847)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242847 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    CROPseq-puro-v2
  • Backbone manufacturer
    Paul Blainey, Addgene plasmid #127458
  • Backbone size (bp) 10224
  • Modifications to backbone
    P2A with mCherry fluorescent protein was inserted downstream of Puromycin resistance gene for double selection.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6
  • Selectable markers
    Puromycin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CROPseq-V2-Puro.P2A.mCherry was a gift from Siyuan Zhang (Addgene plasmid # 242847 ; http://n2t.net/addgene:242847 ; RRID:Addgene_242847)

  • For your References section:

    Tumor-intrinsic IFNγ signaling and niche adaptation drive early colonization in ovarian cancer metastasis. Aleksandrovic E, Fross SR, Golomb SM, Ma W, Liu X, Zhao Z, Das NM, Reese TC, Borges HM, Tenekeci A, Yu L, Lopez J, Zhao M, Zhong Z, Dean KM, Lea J, Xu L, Stack MS, Zhang S. Nat Commun. 2026 Sep 3;17(1):10497. doi: 10.1038/s41467-026-77222-8. 10.1038/s41467-026-77222-8 PubMed 42834039
Related items: