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CCT8-GST
(Plasmid #245887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245887 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEX 4T1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CCT8
  • Species
    H. sapiens (human)
  • Entrez Gene
    CCT8 (a.k.a. C21orf112, Cctq, D21S246, PRED71)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer gst
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The CCT8-GST construct was generated by cloning the CCT8 sequence from the CCT8-pcDNA3.1(+)-N-eGFP plasmid into the pGEX 4T1 vector. The GFP-CCT8 insert was obtained by KpnI digestion, blunted, then cut with XhoI and ligated into the SmaI/XhoI-digested pGEX 4T1 backbone. Please visit https://doi.org/10.64898/2025.12.03.691809 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CCT8-GST was a gift from Guillaume Jacquemet (Addgene plasmid # 245887 ; http://n2t.net/addgene:245887 ; RRID:Addgene_245887)

  • For your References section:

    CCT8 associates with the MYO10 motor domain and regulates filopodia and breast cancer cell invasion. Popović A, Ojalill M, Touma C, Ball NJ, Miihkinen M, Joshi O, Dibus M, Pylvänäinen J, Ivaska J, Goult BT, Jacquemet G. J Cell Sci jcs.265278 10.1242/jcs.265278
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